Separation of Neural Cells Using Two-step Separation by Combination of Soft Inertial Microfluidics and Pinched Flow Fractionation

نویسندگان

  • Zhigang Wu
  • Grzegorz Wicher
  • Åsa Fex Svenningsen
  • Klas Hjort
چکیده

In this study, neural cell sorting of high throughput and high purity is made using two-step separation by combining soft inertial microfluidics and pinched flow fractionation. Both finite element analysis tool and equivalent electric circuits modeling were used to design the microfluidic devices. Separation between neurons and glia cells from rat’s spine cord was demonstrated. To minimize the environmental changes, the aqueous cell culture medium L15 was used in all flow streams.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation.

Blood is a highly complex bio-fluid with cellular components making up >40% of the total volume, thus making its analysis challenging and time-consuming. In this work, we introduce a high-throughput size-based separation method for processing diluted blood using inertial microfluidics. The technique takes advantage of the preferential cell focusing in high aspect-ratio microchannels coupled wit...

متن کامل

Separation of cancer cells from white blood cells by pinched flow fractionation.

In this paper, the microfluidic size-separation technique pinched flow fractionation (PFF) is used to separate cancer cells from white blood cells (WBCs). The cells are separated at efficiencies above 90% for both cell types. Circulating tumor cells (CTCs) are found in the blood of cancer patients and can form new tumors. CTCs are rare cells in blood, but they are important for the understandin...

متن کامل

Separation of Spermatozoa with a Combination of Pinched Flow Fractionation and Tangential Filtration

We demonstrate a pinched flow tangential filtration method to sort spermatozoa from larger particles with a spermatozoa collection efficiency of 94±2% and a separation efficiency of 100%. In conventional pinched flow fractionation (PFF), an observed tumbling-like rotation of spermatozoa complicates their separation from other particles. The new design circumvents this effect.

متن کامل

IB-LBM study on cell sorting by pinched flow fractionation.

Separation of two categories of cells in pinched flow fractionation(PFF) device is simulated by employing IB-LBM. The separation performances at low Reynolds number (about 1) under different pinched segment widths, flow ratios, cell features, and distances between neighboring cells are studied and the results are compared with those predicted by the empirical formula. The simulation indicates t...

متن کامل

Microfluidic Device for Continuous Particle Separation Using Hydrodynamic Filtration

A size-dependent particle separation (classification) is one of the most important procedures in biochemical, environmental, or medical analyses and their applications. However, the smaller particle size or the smaller size difference makes the particle classification difficult. The Lab-on-a-Chip concept have encouraged the miniaturization of various separation or selection methods for small pa...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2010